Libraries can be screened to identify GFP insertions that maintain target protein function at native expression levels, allowing more trustworthy localization studies. The resulting gfp insertions maintain target gene reading frame (to the 5′ and 3′ of gfp) and are integrated at the native chromosomal locus, thereby maintaining native expression signals. TAGIT is a modified Tn5 transposon that uses Kan R to select for insertions on the chromosome or plasmid, β-galactosidase to identify in-frame gene fusions, and Cre recombinase to excise the kan and lacZ genes in vivo. Web site will assume no responsibilities for damages or injuries resulting fromĢ022 (formerly owwm.We constructed a transposon ( transposon assisted gene insertion technology, or TAGIT) that allows the random insertion of gfp (or other genes) into chromosomal loci without disrupting operon structure or regulation. Good judgment and to safely operate old machinery. Themselves may not meet modern standards. Please remember that safety standards haveĬhanged over the years and information in old manuals as well as the old machines On this site nor do we represent any manufacturer listed on this site in any way.Ĭatalogs, manuals and any other literature that is available on this site is madeĪvailable for a historical record only. does not provide support or parts for any machines Our purpose is to provide information about vintage machinery that is generallyĭifficult to locate. PLEASE NOTE: was founded as a public service toĪmateur and professional woodworkers who enjoy using and/or restoring vintage machinery. On our site and Vintage Machinery will receive a small commission from your purchase. Purchase something from or Highland Woodworking, please use the search box here While we do not charge for our services, it does cost us a considerableĪmount of money to bring this site to you. If you find the Vintage Machinery site useful, please consider making a Donation to help offset the cost of bringing this material Visitors are alsoĮncouraged to ask questions and participate in discussion of vintage machinery at Quick Likes below to submit new content to the site:Īnswers to common questions can often be found on the Vintage Machinery Knowledge Base/Wiki. Web site? If so, please read our Submitting site using the power of the Google Search Engine!ĭo you have information or publications that you would like to share with the Recent Manufacturers Index Image Submissions Web Site through our RSS Feeds! Subscribe to the feeds you want and you canĮasily track the most recent content submitted to the Keep track of the latest content submission to the They must be at least 20 years old and manufactured in the U. Vises have now been added to the list of acceptable entries in Vintage Machinery. Serial number tables have been created in the PRODUCTS tab. South Bend Lathe/Machinery serial numbers Thanks to our new volunteer historian David Lundqvist, we now include machinery made in Sweden. Restoration and use of vintage machinery in theĪnd Sell vintage machinery using the FREE Vintage Machinery Classified Ads Owned and restored by our members in the Photo Index Of historical catalogs, manuals and other documents in Publication Reprints Steam and gas engines in North America in the Web site contains information concerning vintage machinery including:Įvery known manufacturer of wood-working and metal-working machinery as well as Compiled through the generousĬontributions of content by thousands of dedicated members, the Vintage Machinery Restoration and use of vintage woodworking machinery. The web site is devoted to information on the history,
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